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D4036 - D4037 -D4019 -D4020 - The fastest, easiest, and purest miniprep kit available for purifying transfection quality plasmid DNA.
Pellet-free procedure omits conventional cell pelleting and resuspension steps. DNA quality appropriate for cloning, sequencing, and transfection. Color-coded reagents for easy visualization and assessment of bacterial cell lysis
The Zyppy™ Plasmid DNA purification kits allow the user to separate plasmid DNA efficiently from chromosomal DNA and cellular RNA in bacterial host cell lysates using procedures that are fast, user friendly, and reliable when compaired to those offered by other suppliers. All of our Zyppy kits include color-coded reagents that allow for easy visualization and assessment of bacterial cell lysis and buffer neutralization steps. Also available is the Zymoprep line of miniprep kits that allow the user to isolate plasmid DNA from yeast.
The Zyppy™ Plasmid Miniprep Kit features a pellet-free modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify using the provided Fast-Spin column technology. Additionally, the innovative colored buffers included in the kit permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
The Zyppy™ Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. coli efficiently. The plasmid DNA is of the highest quality, endotoxin-free, and is well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.
| Format | Spin Column |
|---|---|
| Processing Time | 8 min |
| DNA Purity | Plasmid DNA which is well suited for ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. Typical A(260/280) index is ≥1.8. |
| Elution Volume | ≥ 30 µl |
| Plasmid DNA Size | Up to 25 kb |
| Plasmid DNA Yield | Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized. |
| Procedure | Performed at room temperature (15-30°C) using a centrifuge and/or a vacuum manifold. |
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Marguet P, Tanouchi Y, Spitz E, Smith C, You L | Published: 2010
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Brame CJ, Pruitt WM, Robinson LC | Published: 2008
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Damelin LH, Mavri-Damelin D, Klaenhammer TR, Tiemessen CT | Published: 2010 Jun
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DNA yield from Zyppy™ Plasmid Miniprep Kit and a kit from Supplier A. Plasmid DNA (pGEM®) was digested with EcoRI prior to agarose gel electrophoresis. Performed in triplicate. M, ZR 1 kb DNA Marker (Zymo Research) |
Data Sheet (Size: 1.9 MB) Protocol (Size: 588.9 KB) MSDS (Size: 177.3 KB)
Catalogue lecteurs code 2D pour tubes et racks
