Zymoclean Gel DNA Recovery kit

D4001 - D4002 - D4007 - D4008

Ce kit permet l'extraction d'ADN ultra-pur à partir de bandes de gel d'agarose, dans un volume de 6-8µl d'eau pure.

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The Zymoclean™ Gel DNA Recovery Kit provides for the rapid purification of high quality DNA from TAE/TBE-buffered agarose gels. The product features Fast-Spin technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean™ Gel DNA Recovery kit are perfectly suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc.

Specifications

Format	Spin Column
DNA Recovery	Typically, up to 5 µg total DNA per column can be eluted with ≥6 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.
Processing Time	15 min
Equipment	Microcentrifuge
DNA Size Limits	50 bp to 23 kb
Sample Sources	DNA excised from TAE/TBE-buffered agarose gels.
DNA Purity	High quality, purified DNA is eluted with water which is especially well suited for sequencing, ligation reactions, PCR, labeling, and restriction endonuclease digestions.
Product Detergent Tolerance	≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS

Related PubMed Citations

ATM regulates Mre11-dependent DNA end-degradation and microhomology-mediated end joining...
Rahal EA, Henricksen LA, Li Y, Williams RS, Tainer JA, Dixon K | Published: 2010 Jul 15

Tay1 Protein, a Novel Telomere Binding Factor from Yarrowia lipolytica...
Kramara J, Willcox S, Gunisova S, Kinsky S, Nosek J, Griffith JD, Tomaska L | Published: 2010 Dec 3

Chromosomal reinsertion of broken RSS ends during T cell development...
Curry JD, Schulz D, Guidos CJ, Danska JS, Nutter L, Nussenzweig A, Schlissel MS | Published: 2007 Oct 1

DNA fragments recovered from an agarose gel using the Zymoclean™ Gel DNA Recovery Kit. Lanes: M: DNA Ladder; 1-5: individual ladder DNA fragments.

DNA sequencing chromoatogram of a PCR product recovered using the Zymoclean™ Gel DNA Recovery Kit. DNA was recovered from a 2% (w/v) agarose gel and used directly for sequencing.