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ADN Méthylé Universel Standard SOURIS et Primers de Controle

DNA completely methylated at CpG dinucleotides by CpG Methylase.
Compatible with the EZ DNA Methylation™ family of products and other bisulfite conversion protocols.
Each standard is provided with primer set to amplify a fragment of DNA after bisulfite conversion.



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264,00 € HT

The Universal Methylated Mouse DNA Standard is designed for use as control to assess the efficiency of bisulfite-mediated conversion of DNA in combination with the EZ DNA Methylation™, EZ DNA Methylation- Gold™, or EZ DNA Methylation-Direct™ kits. The control DNA has been enzymatically modified in vitro with CpG Methylase, resulting in methylation at all cytosines in the dinucleotide sequence 5"²...CpG...3"². The methylated cytosines remain unconverted following bisulfite treatment, whereas non-methylated cytosines are converted into uracils and detected as thymines following PCR. Each primer set has been specifically designed to amplify a fragment of the supplied DNA following bisulfite treatment.

Specifications

FormatMale Genomic DNA
Concentration250 ng/µl

 

Related PubMed Citations

Identification of preferential target sites for human DNA methyltransferases...
Choi SH, Heo K, Byun HM, An W, Lu W, Yang AS | Published: 2011 Jan

Quantitative analysis of DNA methylation at all human imprinted regions reveals preservation of...
Woodfine K, Huddleston JE, Murrell A | Published: 2011

Methylated DNA Immunoprecipitation and Microarray-Based Analysis: Detection of DNA Methylation in...
Weng YI, Huang TH, Yan PS | Published: 2009

 



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