E. coli Non-methylated Genomic DNA
250 ng/µl in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)
ADN ELISA adsorption absorption stockage méthylation epigenetique ADN génomique conversion au bisulfite fluorescence sang plasma sérum 384 puits plasmide noire purification ADN sequençage illumina miniprep fond conique culot bactérien microplaque Schleicher et Schüll kit expression baculovirus 96 puits chambre hybridation insecte whatman
250 ng/µl in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)
| Concentration | 250 ng/µl in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) |
|---|---|
| Applications | Negative control for epigenetic experiments requiring DNA containing zero background levels of cytosine and adenosine methylation. Substrate for DNA methylation-sensitive restriction enzyme digestions. |
| Genotype | ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2 |
| Source | Escherichia coli strain ER2925* (Dam"“ and Dcm"“) [*Used with permission from New England Biolabs.] |