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ZR-96 Quick-gDNA™

D3010 - D3011 - D3012 Simple, high throughput (96-well) purification of DNA from whole blood, plasma, serum, body fluids, buffy coat, lymphocytes, tissue, swabs, or cultured cells in about 30 minutes.

Isolated DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.



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205,00 € HT

The ZR-96 Quick-gDNA™ features a simple, high throughput (96-well) procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. The product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, plasma, buffy coat, solid tissue, bone marrow and buccal cells, cells from culture, and many biological liquid samples. For processing, simply add the specially formulated Genomic Lysis Buffer to the samples, vortex, and transfer the mixtures to the wells of the supplied Silicon-A™ Plate. The product yields high-quality, purified DNA in just minutes, and PCR inhibitors are effectively removed during the purification process. Eluted DNA is suitable for PCR, nucleotide blotting, DNA sequencing, endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.

Specifications

Format96-well Plate
DNA RecoveryTypically, the DNA binding capacity of the plate is 5 µg/well. DNA is eluted into ≥ 30 µl DNA Elution Buffer or water. Human whole blood will yield 33 ng - 66 ng DNA per µl blood sampled.
Processing Time30 min
EquipmentCentrifuge w/ microplate carriers.
DNA Size LimitsCapable of recovering genomic DNA > 40 kb. In most instances, mitochondrial DNA is also recovered.
Sample SourcesWhole blood, plasma, or serum from humans, mice, rats, etc. Also, tissue, cells from culture, buccal cells, as well as a variety of liquid samples are effectively processed using this kit.
DNA PurityHigh-quality DNA is eluted with DNA Elution Buffer or water that is especially well suited for PCR and other downstream applications. Typical absorption indices are A(260/280nm) >1.8.
Product Detergent Tolerance≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS

 

Related PubMed Citations

Production of p53 gene knockout rats by homologous recombination in embryonic stem cells...
Tong C, Li P, Wu NL, Yan Y, Ying QL | Published: 2010 Sep 9



Genomic DNA isolated from mouse tail snips using the ZR-96 Quick-gDNA™. A total of 30 mouse tail snips were homogenized with Zymo Research's Squisher-8™ then processed using the ZR-96 Quick-gDNA™. About one third of the number of eluted DNAs was then separated in a 0.8% w/v agarose gel (shown in lanes 1 to 30).





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