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D3040 - D3041 - For high quality DNA purification from solid tissues (e.g., tail snips, ear punches, adipose tissue, etc.), whole blood, plasma, serum, buffy coat, lymphocytes, cultured cells, buccal cells, FFPE tissues, semen, hair, and other biological sources.
Combines Proteinase K digestion with innovative Fast-Spin column technology.
The ZR Genomic DNA™-Tissue MicroPrep is a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, parasitic, microbial, viral) from a variety of solid tissues. The product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and are also compatible with inputs including: buffy coat, bone marrow, cells from culture, whole blood (fresh or stored), serum, plasma, and many biological liquid samples. For processing, simply digest the sample with the supplied Proteinase K then add the Genomic Lysis Buffer, vortex, and transfer the mixture to the supplied spin column. PCR inhibitors are effectively removed during the purification process and purified DNA is suitable for downstream applications including: PCR, Southern blotting, DNA sequencing, endonuclease digestion, bisulfite conversion/methylation analysis, etc.
| • | Format: Spin Column |
| • | Sample Sources: Solid tissues (e.g., tailsnips, earpunches, adipose tissue, etc.), whole blood, plasma, serum, buffy coat, lymphocytes, cultured cells, buccal cells, FFPE tissues, semen, hair, and other biological sources are effectively processed using this kit. |
| • | DNA Size: Capable of recovering genomic and mitochondrial DNA sized fragments from 100 bp to ≥ 40 kb. If present, parasitic, microbial, and viral DNA will also be recovered. Typical fragment sizes range from 25 kb – 35 kb. |
| • | DNA Yield: The DNA binding capacity of the column is 5 µg. Typically, mammalian tissues yield: 1 - 3 µg DNA per mg skeletal, heart, and brain tissues and 3 - 5 µg DNA per mg liver, kidney and lung tissues. Human whole blood will yield 3 - 7 µg DNA per 100 µl blood sampled. DNA is eluted into ≥ 10 μl DNA Elution Buffer or water. |
| • | DNA Purity: High quality DNA for PCR, endonuclease digestion, Southern blotting, bisulfite conversion/methylation detection, sequencing, genotyping, etc., is eluted with DNA Elution Buffer or water (A260/A280 ≥1.8). |
| • | Detergent Tolerance: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
| • | Processing Time: 25 min |
| • | Equipment Needed: Water bath or heat block (55°C), microcentrifuge, and vortex. |
High yield/quality DNA is successfully isolated from porcine muscle using the ZR Genomic DNA™-Tissue MiniPrep. Equivalent amounts (25 mg) of muscle tissue were processed using the ZR Genomic DNA™ Tissue MiniPrep after incubation with Proteinase K at 55ºC for the indicated times (in minutes) or overnight (O/N). Equal volumes of eluted DNA were analyzed in a 0.8% (w/v) TAE/agarose/ethidium bromide gel. M: 1 kb ladder (Zymo Research).
Catalogue lecteurs code 2D pour tubes et racks
