ADN ELISA adsorption absorption stockage méthylation epigenetique ADN génomique conversion au bisulfite fluorescence sang plasma sérum 384 puits plasmide noire purification ADN sequençage illumina miniprep fond conique culot bactérien microplaque Schleicher et Schüll kit expression baculovirus 96 puits chambre hybridation insecte whatman
The ZR-96 Genomic DNA™-Tissue MiniPrep provides a simple, high-throughput procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, parasitic, microbial, viral) from a variety of solid tissues. The product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is also compatible with inputs including buffy coat, bone marrow, cells from culture, whole blood (fresh or stored), serum, plasma, and many biological liquid samples. For processing, simply digest the sample with the supplied Proteinase K then add the Genomic Lysis Buffer, vortex, and transfer the mixture to the supplied Silicon-A™ plate. PCR inhibitors are effectively removed during the purification process and purified DNA is suitable for downstream applications including: PCR, Southern blotting, sequencing, endonuclease digestion, bisulfite conversion/methylation analysis, etc.
| • | Format: 96-well Plate |
| • | Sample Sources: Solid tissues (e.g., tailsnips, earpunches, adipose tissue, etc.), whole blood, plasma, serum, buffy coat, lymphocytes, cultured cells, buccal cells, FFPE tissues, semen, hair, and other biological sources are effectively processed using this kit. |
| • | DNA Size: Capable of recovering genomic and mitochondrial DNA sized fragments from 100 bp to ≥ 40 kb. If present, Parasitic, microbial, and viral DNA will also be recovered. Typical fragment sizes range from 25 kb – 35 kb. |
| • | DNA Yield: The DNA binding capacity of the plate is 5 μg/well. Typically, mammalian tissues yield: 1 - 3 µg DNA per mg skeletal, heart, and brain tissues and 3 - 5 µg DNA per mg liver, kidney and lung tissues. Human whole blood will yield 3 - 7 µg DNA per 100 µl blood sampled. DNA is eluted into ≥ 30 μl/well DNA Elution Buffer or water. |
| • | DNA Purity: High quality DNA for PCR, endonuclease digestion, Southern blotting, bisulfite conversion/methylation detection, sequencing, genotyping, etc., is eluted with DNA Elution Buffer or water (A260/A280 ≥1.8). |
| • | Detergent Tolerance: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
| • | Processing Time: 45 min |
| • | Equipment Needed: Water bath or heat block (55°C), microcentrifuge, and vortex. |
Catalogue lecteurs code 2D pour tubes et racks
