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D3020 - D3021 Easy purification of high quality DNA from whole blood, plasma, serum, body fluids, buffy coat, lymphocytes, tissue, swabs or cultured cells.
15 minute processing time using innovative Fast-Spin column technology.
Protocol excludes the use of Proteinase K and organic denaturants.
| Format | Spin Column |
|---|---|
| DNA Recovery | Typically, up to 5 μg total DNA is eluted into ≥ 10 μl (6 μl minimum) DNA Elution Buffer or water. Human whole blood will yield 1.5-3.5 μg DNA per 50 μl blood sampled. Mammalian tissues yield: 1-3 μg DNA per mg skeletal, heart, and brain tissues a |
| Processing Time | 15 min |
| Equipment | Microcentrifuge, vortex. |
| DNA Size Limits | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Sample Sources | Whole blood, plasma, or serum from humans, mice, rats, etc. Also, tissue, cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. |
| DNA Purity | High-quality DNA is eluted with DNA Elution Buffer or water that is especially well suited for PCR and other downstream applications. Typical absorption indices are A(260/280nm) >1.8. |
| Product Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
Efficient Generation of Fully Reprogrammed Human iPS Cells via Polycistronic Retroviral Vector and a...
Zhang Z, Gao Y, Gordon A, Wang ZZ, Qian Z, Wu WS | Published: 2011
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DNA isolated from porcine whole blood using the Quick-gDNA™ MiniPrep. Equivalent amounts (100 µl) of blood were processed without Proteinase K using the Quick-gDNA™ MiniPrep in half the time as compared to the kits from suppliers A and B. Equal volumes of eluted DNA were then analyzed (in duplicate) in a 0.8% (w/v) TAE/agarose/ethidium bromide gel. The size marker "M" is a 1 kb ladder (Zymo Research). |
Data Sheet (Size: 888.4 KB) Protocol (Size: 192.3 KB)
Catalogue lecteurs code 2D pour tubes et racks
