Quick-gDNA™ MiniPrep

D3006 -D3007 - D3024 - D3025 Easy purification of genomic DNA from whole blood, plasma, serum, body fluids, buffy coat, lymphocytes, tissue, swabs or cultured cells

15 minute processing time using innovative Fast-Spin column technology.

Protocol excludes the use of Proteinase K and organic denaturants.

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The Quick-gDNA™ MiniPrep is for the convenient, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), serum, plasma, buffy coat, solid tissue, bone marrow and buccal cells, cells from culture, and many biological liquid samples can be processed with these kits. The product features Fast-Spin column technology for high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is suitable for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.

Specifications

Format	Spin Column
DNA Recovery	Typically, up to 25 µg total genomic DNA is eluted into ≥ 50 µl DNA Elution Buffer or water. Human whole blood will yield 33 ng - 66 ng DNA per µl blood sampled.
Processing Time	15 min
Equipment	Microcentrifuge, vortex.
DNA Size Limits	Capable of recovering genomic DNA >40 kb. In most instances, mitochondrial DNA is also recovered.
Sample Sources	Whole blood, plasma, or serum from humans, mice, rats, etc. Also, tissue, cells from culture, buccal cells, as well as a variety of liquid samples are effectively processed using this kit.
DNA Purity	High-quality DNA is eluted with DNA Elution Buffer or water that is especially well suited for PCR and other downstream applications. Typical absorption indices are A(260/280nm) >1.8.
Product Detergent Tolerance	≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS

Related PubMed Citations

Efficient Generation of Fully Reprogrammed Human iPS Cells via Polycistronic Retroviral Vector and a...
Zhang Z, Gao Y, Gordon A, Wang ZZ, Qian Z, Wu WS | Published: 2011

Complete Genome Sequence of Mycoplasma suis and Insights into Its Biology and Adaption to an...
Guimaraes AM, Santos AP, SanMiguel P, Walter T, Timenetsky J, Messick JB | Published: 2011

DNA isolated from porcine whole blood using the Quick-gDNA™ MiniPrep. Equivalent amounts (100 µl) of blood were processed without Proteinase K using the Quick-gDNA™ MiniPrep in half the time as compared to the kits from suppliers A and B. Equal volumes of eluted DNA were then analyzed (in duplicate) in a 0.8% (w/v) TAE/agarose/ethidium bromide gel. The size marker "M" is a 1 kb ladder (Zymo Research).