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Accueil>Biologie Moléculaire>Extraction d'ADN>ADN plasmidique>ZR Plasmid Miniprep™-Classic

ZR Plasmid Miniprep™-Classic

D4015 - D4016 - D4054  

  • For purification of high quality, endotoxin-free plasmid DNA for restriction endonuclease digestion, DNA sequencing, transformation, cloning, transfection, in vitro transcription reactions, etc.
  • Innovative colored P1, P2, P3 buffers for rapid identification
  • Unique column design: zero buffer retention and low (30ul) elution volume



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115,00 € HT

The ZR Plasmid Miniprep™-Classic is designed for efficient isolation of plasmid DNA from E. Coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol together with a unique Fast-Spin column to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy determination of complete cell lysis and neutralization. The innovative Zymo-Spin™ llN columns yield endotoxin-free plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep™-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.

Specifications

FormatSpin Column
DNA Recovery≥ 30 µl
Processing Time15 min
DNA PurityPlasmid DNA which is well suited for ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. Typical A(260/280) ≥ 1.8.
Plasmid DNA SizeUp to 25 kb.
Plasmid DNA YieldUp to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.
ProcedurePerformed at room temperature (15-30°C) using a centrifuge and/or a vacuum manifold.

 

 

Plasmid products. Restriction Endonuclease digestion of three different plasmids prepared using the ZR Plasmid Miniprep™-Classic, performed in triplicate. M: ZR 1 kb DNA marker (Zymo Research)

Sequence-quality DNA preparations. DNA sequencing chromatogram of plasmid DNA prepared using the ZR Plasmid Miniprep™-Classic.





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