ZR Plasmid Gigaprep™

The ZR Plasmid Gigaprep Kit employs a modified alkaline lysis method in conjunction with DNA binding beads (ZymoBeads™) to isolate high quality endotoxin-free (for transfection) plasmid DNA in less than an hour. The purified DNA is suitable for use in transfection, restriction endonuclease digestion, ligation, bacterial transformation, PCR amplification, DNA sequencing and other sensitive molecular biology applications. The innovative patented colored buffers included in the kit permit error-free visualization of both complete bacterial cell lysis and neutralization. Additionally, the uniquely designed Midi Filter allows the capture of ZymoBeads™ either by centrifugation or vacuum. The unique design of the filter also allows for low elution volumes of 2-3 ml directly in supplied Elution buffer or water, thus eliminating the need for plasmid DNA precipitation and resuspension steps common to other column-based gigaprep procedures. The ZR Plasmid Gigaprep Kit is designed for use with a combination of both centrifuge, and vacuum manifold, or a centrifuge alone, therefore providing flexibility in large scale plasmid DNA purification from E. coli. An overview of the purification procedure is shown below.