Aiguilles de dépôt de cellules entières

Whole Cell Microarray Printing Pins are based on the most widely used microarray manufacturing in the world. These Pins have 100 or 500 µm wide uptake channels to efficiently print intact human, fungal and bacterial cells into microarrays. These microarray printing pins are compatible with Professional, 946 and Stealth style Printheads. For best results use with Professional Series Printhead and NanoPrint or SpotBot Microarrayers. 

Whole Cell Microarray Printing Pins are based on the most widely used microarray manufacturing in the world. These Pins have 100 or 500 µm wide uptake channels to efficiently print intact human, fungal and bacterial cells into microarrays. These microarray printing pins are compatible with Professional, 946 and Stealth style Printheads. For best results use with Professional Series Printhead and NanoPrint or SpotBot Microarrayers.

Reference Publication:
Human Cell Chips: Adapting DNA Microarray Spotting Technology to Cell-Based Imaging Assays
T Hart, A Zhao, A Garg, S Bolusani, EM Marcotte - PLoS ONE, 2009 - dx.plos.org
Abstract: Here we describe human spotted cell chips, a technology for determining cellular state across arrays of cells subjected to chemical or genetic perturbation. Cells are grown and treated under standard tissue culture conditions before being fixed and printed onto replicate glass slides, effectively decoupling the experimental conditions from the assay technique. Each slide is then probed using immunofluorescence or other optical reporter and assayed by automated microscopy. We show potential applications of the cell chip by assaying HeLa and A549 samples for changes in target protein abundance (of the dsRNA-activated protein kinase PKR), subcellular localization (nuclear translocation of NFκB) and activation state (phosphorylation of STAT1 and of the p38 and JNK stress kinases) in response to treatment by several chemical effectors (anisomycin, TNFα, and interferon), and we demonstrate scalability by printing a chip with ~4,700 discrete samples of HeLa cells. Coupling this technology to high-throughput methods for culturing and treating cell lines could enable researchers to examine the impact of exogenous effectors on the same population of experimentally treated cells across multiple reporter targets potentially representing a variety of molecular systems, thus producing a highly multiplexed dataset with minimized experimental variance and at reduced reagent cost compared to alternative techniques. The ability to prepare and store chips also allows researchers to follow up on observations gleaned from initial screens with maximal repeatability.

Image 1: Magnified image of the tip of the whole cell microarray printing pin capillary channel.  Width of the channel is 500 microns.

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License
This product is protected by US Patent Number 6,101,946 and is sold license- and royalty-free for research purposes only.

Based on print tests using the NanoPrint Microarrayer at 50% humidity onto SuperEpoxy 2 microarray substrates.  Pins have 1.25 µm uptake loading volumes.  Number of actual spots can depend on surface chemistry, buffer system and sample type. It is best to empirically test the number of spots in the specific test system being used.