EZ Bisulfite DNA Clean-up Kit™
D5025 - Permet d’éliminer les contaminants issus de réaction de conversion au bisulfite « maison ».
Pas nécessaire si vous avez utilisé les kits Zymo EZ DNA Methylation (classique, GOLD ou DIRECT).
Catégories
- Epigénétique
- Séquençage haut-débit
- Biologie Cellulaire
- Biologie Moléculaire
- Extraction d'ADN
- Purification de fragments d'ADN
- ADN génomique
- ADN plasmidique
- Purification d'ARN
- Microarraying
- Transformation et bactéries compétentes
- Levure
- Consommables
- Extraction d'ADN
- Stockage - Criblage
- Petit Matériel
ADN ELISA absorption stockage adsorption méthylation epigenetique ADN génomique conversion au bisulfite fluorescence sang plasma purification ADN 384 puits plasmide sérum noire sequençage illumina miniprep fond conique culot bactérien microplaque baculovirus 96 puits chambre hybridation insecte kit expression NGS ffpe
Permet d’éliminer les contaminants issus de réaction de conversion au bisulfite « maison ».
Pas nécessaire si vous avez utilisé les kits Zymo EZ DNA Methylation (classique, GOLD ou DIRECT).
Consistent, high yield recovery of bisulfite-treated DNA from any "homebrew" or commercial reaction mixture containing bisulfite.
Simple spin column procedure with small elution volumes (≥ 6 µl) for concentrated DNA.
The EZ Bisulfite DNA Clean-up Kit™ has been specifically designed for the purification of bisulfite-treated DNA from any "homebrew" or commercial reaction mixture containing bisulfite. This product features innovative Fast-Spin, in-column desulfonation and wash technologies that eliminate DNA loss, buffer carryover, and the need for ethanol/isopropanol precipitations. The procedures are easy and DNA clean-up can be completed in just minutes. Bisulfite-treated DNA purified with this product is ideal for PCR amplification for downstream DNA methylation analyses including restriction endonuclease digestion, sequencing, microarrays, etc.
Specifications
| Format | Spin Column |
|---|---|
| DNA Recovery | > 80% |
| Processing Time | 25 min |
| Equipment | Microcentrifuge |
| Binding Capacity | 5 µg/prep |
| Elution Volume | ≥ 10 µl |
| Input | Bisulfite-converted DNA |
Related PubMed Citations
DNA methylation analysis by digital bisulfite genomic sequencing and digital MethyLight...
Weisenberger DJ, Trinh BN, Campan M, Sharma S, Long TI, Ananthnarayan S, Liang G, Esteva FJ, Hortobagyi GN, McCormick F, Jones PA, Laird PW | Published: 2008 Aug
Bisulfite Sequencing of DNA...
Darst RP, Pardo CE, Ai L, Brown KD, Kladde MP | Published: 2010 Jul
A new method for accurate assessment of DNA quality after bisulfite treatment...
Ehrich M, Zoll S, Sur S, van den Boom D | Published: 2007 Mar
Data Sheet (Size: 643.7 KB)- Protocol (Size: 318.1 KB)
- MSDS (Size: 146.8 KB)
