ChIP DNA Clean & Concentrator™

D5201 - D5205 ChIP DNA Clean & Concentrator™

Two (2) minute DNA clean-up from any step in a standard ChIP protocol.
DNA is ideal for PCR, arrays, DNA quantification, Southern blot analysis, sequencing, and other molecular applications.

En savoir plus

The Chromatin Immunoprecipitation (ChIP) DNA Clean & Concentrator™ provides a hassle-free method for the rapid purification and concentration of high quality DNA from any step in a standard ChIP protocol. This includes samples that have undergone reverse cross-linking, Proteinase K or RNase A digestion, mechanical or nuclease-mediated DNA shearing, and samples eluted from chromatin-antibody-bead complexes. The specially formulated ChIP DNA Binding Buffer promotes DNA adsorption to the column in the presence of detergents, antibodies, and proteinases that are often used for ChIP. This kit may be applied to any routine ChIP procedure to determine DNA concentration of samples that have undergone reverse cross-linking following DNA shearing. It can also be used for the removal of TES, 0.1M NaHCO3 and 1% SDS from DNA eluted from chromatin-antibody-bead complexes and can be used to purify DNA from buffers containing up to 1% SDS or 5% NP-40, Tween-20, Triton X-100 or Sarkosyl.

Specifications

DNA Recovery	For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.
DNA Size Limits	50 bp to ~ 23 kb
Sample Sources	Wherever DNA isolation and purification is required during standard ChIP protocols. This includes samples that have undergone reverse cross-linking and Proteinase K or RNase A digestion following either 1) mechanical or nuclease-mediated DNA shearing or 2) elution from chromatin-antibody-bead complexes in TES, 0.1M NaHCO3 and 1% SDS, or other buffers containing up to 1% SDS. This kit can also be used for DNA purification from PCR, enzymatic digestion, kinase, phosphatase and other enzymatic reactions.
DNA Purity	High quality, purified DNA is eluted with elution buffer or water and is especially well suited for PCR amplification, arrays, Southern blot analysis, DNA quantification, sequencing, and other molecular applications.
Product Detergent Tolerance	≤ 5% Triton X-100, ≤ 5% Tween-20, ≤ 5% Sarkosyl, ≤ 1% SDS

Related PubMed Citations

ChIP on Chip: surprising results are often artifacts...
Waldminghaus T, Skarstad K | Published: 2010

Activation of Protein Kinase Tel1 through Recognition of Protein-Bound DNA Ends...
Fukunaga K, Kwon Y, Sung P, Sugimoto K | Published: 2011 May

Sequence-Specific Capture of Protein-DNA Complexes for Mass Spectrometric Protein Identification...
Wu CH, Chen S, Shortreed MR, Kreitinger GM, Yuan Y, Frey BL, Zhang Y, Mirza S, Cirillo LA, Olivier M, Smith LM | Published: 2011

Agarose gel electrophoresis of DNA isolated from cell lysates. High quality DNA can be efficiently recovered from Saccharomyces cerevisiae cell lysates using the ChIP DNA Clean & Concentrator™. Duplicate purifications were performed with 5, 15, and 30 µl cell lysate and an equal volume of eluted DNA was loaded into each lane. The size marker M1 and M2 are 100 bp and 1 kb ladders, respectively (Zymo Research).