ADN Méthylé Standard HUMAIN

D5011 - L'ADN méthylé universel standard comprend l'ADN méthylé enzymatiquement ainsi qu' un ensemble de primers spéciallement conçus pour être utilisés avec les kits de conversion au bisulfite .

Plus de détails

En savoir plus

DNA completely methylated at CpG dinucleotides by CpG Methylase. Compatible with the EZ DNA Methylation™ family of products and other bisulfite conversion protocols.

Each standard is provided with primer set to amplify a fragment of DNA after bisulfite conversion.

The Universal Methylated Human DNA Standard is designed for use as control to assess the efficiency of bisulfite-mediated conversion of DNA in combination with the EZ DNA Methylation™, EZ DNA Methylation- Gold™, or EZ DNA Methylation-Direct™ kits. The control DNA has been enzymatically modified in vitro with CpG Methylase, resulting in methylation at all cytosines in the dinucleotide sequence 5"²...CpG...3"². The methylated cytosines remain unconverted following bisulfite treatment, whereas non-methylated cytosines are converted into uracils and detected as thymines following PCR. Each primer set has been specifically designed to amplify a fragment of the supplied DNA following bisulfite treatment.