Fabricants

RNA/DNA/Protein Purification Plus Micro Kit (Cat. 51600)

RNA/DNA/Protein Purification Plus Micro Kit (Cat. 51600)

RNA/DNA/Protein Purification Plus Micro Kit (Cat. 51600)

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662,00 € HT

51600

pdf51600 - Protocol (50 prep)

 

 

RNA/DNA/Protein Purification Plus Micro Kit Figure 1

RNA/DNA/Protein Purification Plus Micro Kit Figure 1

For sequential isolation of total RNA, genomic DNA and total proteins from the same sample

 
  • Sequentially purify RNA (and miRNA), DNA and proteins from a single sample
  • Small elution volume down to 20 µL with a specialized column
  • No sample splitting, no need to use phenol or precipitation methods
  • Proteins are purified on column and are soluble in the elution buffer
  • Proteins require no further cleaning - ready for Western blot and Mass spectrometry
  • Suitable for cells, tissues, stem cells, CTC, small input of samples
  • Rapid and efficient spin column procedure - all done in 30 minutes

 

Kit Specifications
Maximum Column Binding Capacity
35 μg for RNA
10 μg for DNA
100 μg for protein
Maximum Column Loading Volume
650 μL
Size of RNA Purified
All sizes, including
small RNA (< 200 nt)
Size of DNA Purified
≥ 30 kb
Time to Complete 10 Purifications
30 minutes
Maximum Amount of Starting Material:
Animal Cells
Animal Tissues
Blood
Plant / Fungi Tissues
 
5 x 105 cells
5 mg (for selected tissues)
50 µL
25 mg
Average Yields*
HeLa Cells (5 x 105 cells)
HeLa Cells (5 x 105 cells)
HeLa Cells (5 x 105 cells)
RNA: 7.5 μg
DNA: 1-2 μg
Protein: 70 μg

 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers. The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT).

 

Kit Components
ComponentCat. 51600 (50 preps)
Buffer SKP40 mL
Wash Solution A2 x 38 mL
Elution Solution A6 mL
Wash Solution E15 mL
Elution Buffer F15 mL
Wash Solution C30 mL
Binding Buffer A8 mL
Elution Buffer C8 mL
Protein Neutralizer4 mL
Protein Loading Dye2 mL
gDNA Purification Columns50
RNA/Protein Purification Micro Columns50
Collection Tubes150
Elution Tubes (1.7 mL)150
Product Insert1
 
 
 
 

 

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