Fabricants

RNA/DNA/Protein Purification Plus Micro Kit (Cat. 51600)

RNA/DNA/Protein Purification Plus Micro Kit (Cat. 51600)

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662,00 € HT

51600

pdf51600 - Protocol (50 prep)

 

 

RNA/DNA/Protein Purification Plus Micro Kit Figure 1

RNA/DNA/Protein Purification Plus Micro Kit Figure 1

For sequential isolation of total RNA, genomic DNA and total proteins from the same sample

 
  • Sequentially purify RNA (and miRNA), DNA and proteins from a single sample
  • Small elution volume down to 20 µL with a specialized column
  • No sample splitting, no need to use phenol or precipitation methods
  • Proteins are purified on column and are soluble in the elution buffer
  • Proteins require no further cleaning - ready for Western blot and Mass spectrometry
  • Suitable for cells, tissues, stem cells, CTC, small input of samples
  • Rapid and efficient spin column procedure - all done in 30 minutes

 

Kit Specifications
Maximum Column Binding Capacity
35 μg for RNA
10 μg for DNA
100 μg for protein
Maximum Column Loading Volume
650 μL
Size of RNA Purified
All sizes, including
small RNA (< 200 nt)
Size of DNA Purified
≥ 30 kb
Time to Complete 10 Purifications
30 minutes
Maximum Amount of Starting Material:
Animal Cells
Animal Tissues
Blood
Plant / Fungi Tissues
 
5 x 105 cells
5 mg (for selected tissues)
50 µL
25 mg
Average Yields*
HeLa Cells (5 x 105 cells)
HeLa Cells (5 x 105 cells)
HeLa Cells (5 x 105 cells)
RNA: 7.5 μg
DNA: 1-2 μg
Protein: 70 μg

 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers. The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT).

 

Kit Components
ComponentCat. 51600 (50 preps)
Buffer SKP 40 mL
Wash Solution A 2 x 38 mL
Elution Solution A 6 mL
Wash Solution E 15 mL
Elution Buffer F 15 mL
Wash Solution C 30 mL
Binding Buffer A 8 mL
Elution Buffer C 8 mL
Protein Neutralizer 4 mL
Protein Loading Dye 2 mL
gDNA Purification Columns 50
RNA/Protein Purification Micro Columns 50
Collection Tubes 150
Elution Tubes (1.7 mL) 150
Product Insert 1
 
 
 
 

 

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