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D4048 - D4049 - ZR BAC DNA Miniprep Kit
The ZR BAC DNA Miniprep Kit is for the efficient isolation of BAC plasmid DNA or other large plasmids (e.g., PAC) from E. coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization and assessment of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.
| Format | Spin Column |
|---|---|
| Processing Time | 15 min |
| DNA Purity | High-purity and endotoxin-free (<50 EU/μg) plasmid DNA in low salt buffer or water; typical A260/280 ≥ 1.8. Eluted DNA is suitable for sequencing, endonuclease digestion, PCR, and other reactions requiring highly purified DNA. |
| Plasmid DNA Size | 50 bp to 200 kb. |
| Plasmid DNA Yield | Up to 10 μg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed,and culture growth conditions. The typical yield is 1 μg per 3 ml of culture. |
| Procedure | Can be conducted at room temperature, between 15-30°C. |
| Recovery Volume | ≥ 10 µl |
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