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D3074 - Quick method for the purification of high quality DNA from up to 3 ml whole blood, plasma, and serum, in less than 20 minutes using innovative Fast-Spin column technology. Up to 125 µg/prep
Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).
Unique extraction technology excludes the use of Proteinase K and organic denaturants.
The Quick-gDNA™ Blood MidiPrep is a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, and plasma. Isolated DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
| • | Format: Spin Column |
| • | Sample Sources: Up to 3 ml (see protocol) whole blood, plasma, or serum from humans, mice, rats, etc. |
| • | DNA Size Limits: Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| • | DNA Recovery: Up to 125 µg total DNA is eluted into ≥150 µl DNA Elution Buffer or water. Human whole blood will typically yield 3-7 µg DNA per 100 µl blood sampled. |
| • | DNA Purity: High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8 |
| • | Product Detergent Tolerance: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS. |
| • | Processing Time: 20 min |
| • | Equipment Needed: Centrifuge or vacuum source and manifold, microcentrifuge, vortex |
Catalogue lecteurs code 2D pour tubes et racks
