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Zymoclean™ Large Fragment DNA Recovery Kit

D4045 - D4046 - Zymoclean™ Large Fragment DNA Recovery Kit

  • Quick (20 minute) high-throughput recovery of ultra-pure DNA from agarose gels.
  • Column design permits DNA elution at high concentrations into minimal volumes (≥ 15 µl).
  • Eluted DNA is well suited for use in DNA ligation, sequencing, labeling, PCR, etc.



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83,00 € HT

The Zymoclean™ Large Fragment DNA Recovery Kit provides a streamlined method for the rapid purification and concentration of high-quality large-sized DNA from agarose gels. Simply add the specially formulated Agarose Dissolving Buffer (ADB) to the gel slice containing a DNA sample, let dissolve, and then transfer to the supplied Zymo-Spin™ IC-XL Column. There is no need for organic denaturants or chloroform. Instead, the product utilizes unique spin column technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean™ Large Fragment DNA Recovery Kit is ideal for PCR, sequencing, endonuclease digestion, ligation, etc. The entire procedure typically takes about 15 minutes.

Specifications

FormatSpin Column
DNA RecoveryTypically, up to 10 μg total DNA per column can be eluted into ≥ 10 μl water. For DNA 50 bp to 23 kb the recovery is 70-90%. For DNA ≥ 23 kb, the recovery is 50-70%.
EquipmentMicrocentrifuge
DNA Size Limits50 bp to ~200 kb
Sample SourcesDNA excised from TAE and TBE-buffered agarose gels.
DNA PurityHigh-quality, purified DNA is eluted with water making it especially well suited for sequencing and ligation reactions.
Product Detergent Tolerance≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS

 

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Recovery of large DNA fragments. The Zymoclean™ Large Fragment DNA Recovery Kit was used to recover ? DNA digested with HindIII and separated by agarose gel electrophoresis. Lane C: ?-HindIII digest; lanes 1 & 3: recovered 23 kb ?-HindIII fragments; lanes 2 & 4: recovered 9 kb ?-HindIII fragments. Lane ?: intact ? phage DNA; lanes 5, 6: intact ? ~48 kb bands.





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