Zyppy™ Plasmid Maxiprep Kit

The Zyppy™ Plasmid Maxiprep Kit employs a modified alkaline lysis method in conjunction with spin-column purification to isolate high quality, endotoxin-free plasmid DNA in minutes. The innovative colored buffers included in the kit permits error-free visualization identification of complete bacterial cell lysis and neutralization. Additionally, the uniquely designed Zymo-Maxi Filter™ column permits lysate clearing without centrifugation while the high capacity DNA-binding Zymo-Spin™ VI column allows for low 2 - 3 ml elution volumes, eliminating the need for DNA precipitation and resuspension steps common to other column-based maxiprep procedures. The purified DNA is suitable for use in transfection, restriction endonuclease digestion, ligation, bacterial transformation, PCR amplification, sequencing, and other sensitive downstream applications. The Zyppy™ Plasmid Maxiprep Kit is designed for use with a centrifuge, a vacuum manifold, or a combination of both procedures and provides the greatest flexibility in large scale plasmid DNA purification from E. coli. An overview of the purification procedure is shown below.