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D3100 - For the purification of high quality DNA from up to 3 ml whole blood, plasma, serum, body fluids, buffy coat, lymphocytes, cultured cells, and tissues.
Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).
Eluted, inhibitor-free DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
The Quick-gDNA™ MidiPrep is a large-scale version of the Quick-gDNA™ MicroPrep and Quick-gDNA™ MiniPrep kits. It provides a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources, including whole blood (fresh or stored), serum, plasma, buffy coat, solid tissue, bone marrow and cells from culture, and many biological liquid samples. The specially formulated Genomic Lysis Buffer removes the need for organic denaturants or Proteinase K digestion. High quality DNA is purified in just 20 minutes using Fast-Spin column technology incorporated into the Zymo-Spin™ V-E Column w/ Zymo-Midi Filter™. DNA purified using the Quick-gDNA™ MidiPrep is suitable for PCR, nucleotide blotting, sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
| • | Format: Spin Column |
| • | Sample Sources: Up to 3 ml (see protocol) whole blood, plasma, or serum from humans, mice, rats, etc. Also, tissue, cells from culture, as well as a variety of biological liquids are effectively processed using this kit. |
| • | DNA Size Limits: Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| • | DNA Recovery: Typically, up to 125 µg total DNA is eluted into ≥ 150 µl DNA Elution Buffer or water. Human whole blood will yield 3-7 µg DNA per 100 µl blood sampled. Mammalian tissues yield: 1-3 µg DNA per mg skeletal, heart, and brain tissues and 3-5 µg DNA per mg liver, kidney and lung tissues. |
| • | DNA Purity: High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. Typical absorption indices are A260/A280 >1.8. |
| • | Detergent Tolerance: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS. |
| • | Processing Time: 30 min |
| • | Equipment Needed: Centrifuge or vacuum source and manifold, microcentrifuge, vortex. |
Catalogue lecteurs code 2D pour tubes et racks
